Multi-Parametric Surface Plasmon Resonance (MP-SPR)

Surface Plasmon Resonance

The principle of Surface Plasmon Resonance (SPR) is based on the behavior of electrons at the surface of a metal when light hits it at a certain angle. These electrons start to oscillate together, creating what is called a surface plasmon wave. This happens very specifically depending on the angle of the light.

When molecules bind to the sensor surface, they change how light is reflected, which shifts the angle where this resonance occurs. By measuring this shift in real-time, scientists can determine how much of the molecule has bound to the surface and study the interactions between different molecules.

Multi-Parametric Surface Plasmon Resonance (MP-SPR) is built upon the principles of SPR theory. Its advantageous goniometric optical arrangement allows it to measure more parameters than traditional SPR instruments, providing a complete SPR curve.

Complete SPR curve data is beneficial in all interaction measurements and it is delivering crucial information for:

  • Live cell measurements
  • Crude sample measurements
  • Layer characterization
  • Gas measurements
  • Reference channel-free operations

Key benefits of MP-SPR

  • Precise and high content data
  • Real-time, label-free measurements of molecular interactions, including affinity and kinetics
  • Thickness characterization, from Ångströms to several micrometers
  • Effective in both gas and liquid environments
  • Suitable for complex targets like nanoparticles, viruses, and live cells
  • Compatible with various media, including cell cultures and serum
  • Equipped with a wide range of unique surfaces
  • Possibility to combine with fluorescence and electrochemistry with dedicated flow-cells
  • Flexible design, possible to combine with other analytical techniques
  • Easy to use instrumentation

Read more about MP-SPR technology