Is MP-SPR the best technique to study extracellular vesicles (EVs)?

MP-SPR has proved itself to be a highly effective way to measure and study extracellular vesicles (EVs). What is it and how can you use the technique in your research? Read more and find out!

What is MP-SPR?

MP-SPR stands for Multi-Parametric Surface Plasmon Resonance. It is an excellent surface measurement technique developed by BioNavis Ltd. It has various use cases, such as measuring interactions with live cells, nanoparticles, layer properties, and molecular interactions to name a few.

Compared to traditional SPR, MP-SPR has some major advantages when it comes to studying nanoparticles such as EVs. Unlike MP-SPR, traditional SPR is not designed for nanoparticles and therefore, even the most sensitive and top-tier instruments might clog if nanoparticles are used.

Also, MP-SPR has a unique optical setup that allows us to measure the full SPR curve simultaneously with multiple wavelengths. This feature exceeds the measurement capacity of traditional SPR and provides a lot of essential information, such as total internal reflection angle (TIR), peak minimum angle, and intensity.


Studying EVs with MP-SPR

Studies on extracellular vesicles show real potential in the medical field. In health-related diagnostics, EVs are found to be very interesting due to their many characteristics.

BioNavis’ MP-SPR instruments solve multiple challenges when it comes to studying EVs: our instruments are able to measure all sizes of EVs without bulk effect affecting the results.

The methods currently used in EV studies are, while powerful for their own purposes, not able to measure nanoparticles that are smaller than 100nm in size, such as platelet EVs. BioNavis’ MP-SPR instruments can be used to measure all EV sizes, even the smallest ones!

Bulk effect is an undesired shift in the measured SPR curve caused by differences in the samples’ liquid composition or running buffer. The effect is present with any method recording refractive index changes close to a sensor surface. However, by measuring the full SPR curve, BioNavis’ instruments are able to utilize cross-correlation of parameters to calculate out the bulk effect (PureKinetics™).

BioNavis offers support and high-quality instruments for researchers

BioNavis has developed high-quality MP-SPR instruments for over 10 years. By constantly staying ahead of the latest developments, we are able to provide technology that goes beyond today’s capabilities.

The MP-SPR technology can be used for many purposes, such as finding the optimal EVs for drug delivery or other nanoparticle research.

Out of all our instruments, MP-SPR Navi™ 220A NAALI and MP-SPR Navi™ 420A ILVES are the best ones for studying EVs. These models also require the smallest sample volumes.

As a company, we are enthusiastic about providing the best tools for your research – we offer support in all the different ways our instruments can be used to study EVs and other nanoparticles. Our MP-SPR instruments are flexible to use and can easily be adapted to suit your research.

Peer-reviewed publications:


Read more about our MP-SPR instruments and feel free to contact us!
We can give you more information about how MP-SPR suits your study.

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