AN#177 Simultaneous Real-Time Fluorescence and MP-SPR Measurements

This application note presents simultaneous measurements of fluorescence and MP-SPR during the layer-by-layer assembly of polyelectrolyte multilayers (PEMs; case 1) labeled with Cy5.5 dye and interaction of Alexa Fluor700- labeled antigen with a surface immobilized antibody (case 2). In the case of PEMs, fluorescence data indicates the influence of fluorophore distance from the gold surface on signal intensity, while MP-SPR tracks thickness changes in real time. The simultaneously recorded antigen-antibody fluorescence and MP-SPR data reveal similar concentration-dependent binding.

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