AN#176 Size and Concentration Measurement of Extracellular Vesicles Using MP-SPR
This Application Note demonstrates the use of Multi- Parametric Surface Plasmon Resonance (MP-SPR) for simultaneous size and concentration measurements of extracellular vesicles (EVs) derived from COLO-1 cells. MP-SPR quantifies EV capture at the sensor surface and extracts both size and bulk concentration through dualwavelength operation. The approach builds upon validated methodologies where dual-wavelength detection resolves vesicle deformation effects and improves quantification accuracy. The regenerable sensor surface allows repeated measurements, enhancing throughput and reducing assay costs.
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