AN#175 Monitoring Protein Corona Formation on Extracellular Vesicles Using MP-SPR
Application Note #175
This Application Note demonstrates how Multi-Parametric Surface Plasmon Resonance (MP-SPR) measures protein corona formation on extracellular vesicles (EVs) derived from COLO-1 cells when exposed to human plasma (Figure1). MP-SPR quantifies the adsorption and partial removal of the protein corona in real-time using buffer and detergent washes. Dual-wavelength measurements enable direct determination of EV and corona layer thickness throughout the interaction.