Regenerable avidin kit assays Part II: Biomolecule affinity and concentration studies

Application Note #168

Binding of different PIPs binding to ORP2.

MP-SPR Navi™ Regenerable avidin kit is an excellent tool in development of MP-SPR based assays. Here, the specific binding affinity of lipid based molecules towards sensor-captured protein was analyzed. Also, concentration analysis of diagnostically relevant biomarkers were performed in human serum samples. Utilizing Regenerable avidin kit reduces time in assay development since there is no need to do traditional immobilization conditions optimization which is typically needed for covalent immobilization over dextran sensor slides.

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