Affinity and kinetics of extracellular vesicles – protein interaction

Application Note #164

Affinity and kinetic constants of peptide – extracellular vesicle interaction was determined using TraceDrawer™ for MP-SPR Navi™. The solid black line shows measured data, and the dashed orange line represents the fit to sensogram from sequential injections of three different populations of EVs having A) 1%, B) 5%, and C) 10% coverage of α3β1 integrin.

Extracellular vesicles (EVs) are extensively studied as potential therapeutics, drug delivery systems and for non-invasive diagnostics of diseases, such as cancer. Early detection of ovarian cancer was explored using Multi-Parametric Surface Plasmon Resonance (MP-SPR) instrument. Tumor EVs (SKOV-3) were loaded onto surface-immobilized LXY30 peptide. Affinity and kinetic constants of the interaction, bound mass and layer thickness were determined based on MP-SPR measurement. LXY30 peptide exhibits a high affinity to EVs expressing α3β1 integrin and offers possibility for diagnostic applications.

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