Concentration analysis of nutritionally important proteins from milk
Application Note #162
Concentrations of proteins were determined from milk and milk-based infant formula using Multi-Parametric Surface Plasmon Resonance (MP-SPR) instrument. α-Lactalbumin (ALAB) is a nutritionally important protein for infants, and immunoglobulin G (IgG) is an important part of the secondary immune response. Concentrations of unknown samples were determined based on calibration curves. The assay was a direct binding assay using an anti-ALAB antibody or anti-IgG immobilized on the surface. The same assay format can be used to detect other similar proteins of interest such as antibiotic residues, toxins, allergens or nutrients.