Nanoparticle uptake by cells measured using MP-SPR

Application Note #156

Uptake of mesoporous silica nanoparticles in human epithelial cervical cancer cells (HeLa). Positively charged nanoparticles (C-SiNPs) showed a more efficient uptake and caused a larger response in MP-SPR than negatively charged NPs (P-SiNPs). There was a rapid initial response when SiNPs reached the flow-cell, due to some remaining stock solvent dimethyl sulfoxide (DMSO) after dilutions.

Nanoparticles (NPs) are extensively studied as drug delivery systems. NPs enter the cells usually by active transport, i.e. endocytosis. Multi-Parametric Surface Plasmon Resonance (MP-SPR) is previously used for protein-protein interactions is now used in pioneering NP – living cell studies. Confluent monolayers of human epithelial cervical cancer cells (HeLa) were grown on sensor slides. Uptake of mesoporous silica nanoparticles (SiNPs), branched polyethyleneimine–DNA polyplexes (bPEI–DNA PPs), and extracellular vesicles (EVs) were studied using MP-SPR. Uptake was measured at different temperatures and the activation energy of the cell uptake was calculated using Arrhenius plots.

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