Drug delivery nanocarrier studies using MP-SPR

Application Note #152

(A) Multi-Parametric Surface Plasmon Resonance (MP-SPR) measuring the binding of nanoparticles to surface immobilized molecules: Binding of block copolymer micelles (LMP) to mucin surfaces was studied for muco-adhesive ocular drug delivery. (B) Binding of molecules to surface attached nanoparticles: Binding of the opsonizing agent C3b to modified liposome-based nanoparticles was studied to assess nanocarrier immune response.

Nanocarriers are being extensively studied as drug delivery systems for cancer and gene therapy amongst others. Block copolymeric micelles (LMP) were developed as mucoadhesive ocular drug delivery vehicles. Micelles and chitosan interaction parameters on mucin surface were determined using Multi-Parametric Surface Plasmon Resonance (MP-SPR). Micelles adhesion was found to increase slightly with increasing phenylboronic acid (PBA) content of the nanoparticle.

In a separate study, binding of C3b molecules to liposomes was measured using MP-SPR. C3b is part of the innate immune system and it serves as an opsonizing agent enhancing phagocytosis (more specifically tagging the cell for phagocytosis). The affinity of C3b on PEGylated oligo-guanidyl lipid modified (OGP + PEG) liposomes was KD = 7.2 × 10−8 M and surface mass density Γ = 120 – 200 ng/cm2. The binding level was higher on liposomes without PEG.

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