Virus interaction studies using MP-SPR

Application Note #145

Peptides PolyK-SIINFEKL and SIINFEKL interaction with oncolytic adenoviruses (OAds).

To develop cancer vaccines, a virus-peptide interaction was measured with Multi-Parametric Surface Plasmon Resonance (MP-SPR). Viruses were coated with peptides in order to increase virus immunogenicity. Oncolytic adenoviruses (OAds) were attached on the sensor surface. Tumor-specific major histocompatibility complex (MHC) peptides attachment to the OAds was measured in real-time. Modified positive polyK-SIINFEKL peptide bound to the virus surface (binding constant 2.88 x 10 M), whereas unmodified neutral SIINFEKL peptide did not bind.

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