Supported lipid layers characterization with MP-SPR

Application Note #139

Change in SPR peak minimum angle during lipid deposition, From left to right: SLB formation on SiO2 surface, SLV formation on PEG-SAM surface and SLB formation on 6 kDa dextran surface.

Multi-Parametric Surface Plasmon Resonance (MP-SPR) was used to characterize lipid layer structures adsorbed on a different substrates. Silicon dioxide and low molecule weight dextran surfaces supported formation of lipid bilayer (SLB) whereas thiolated polyethylene glycol supported vesicular layer formation (SLV). Thickness and refractive index of deposited lipid layer was calculated: SLB was about 5 nm thick layer whereas SLV was 10 nm thick layer.

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