Triggering Protein Adsorption on Tailored Cationic Cellulose Surfaces

The equipment of cellulose ultrathin films with BSA (bovine serum albumin) via cationization of the surface by tailor-made cationic celluloses is described. In this way, matrices for controlled protein deposition are created, whereas the extent of protein affinity to these surfaces is controlled by the charge density and solubility of the tailored cationic cellulose derivative. In order to understand the impact of the cationic cellulose derivatives on the protein affinity, their interaction capacity with fluorescently labeled BSA is investigated at different concentrations and pH values. The amount of deposited material is quantified using QCM-D (quartz crystal microbalance with dissipation monitoring, wet mass) and MP-SPR (multi-parameter surface plasmon resonance, dry mass), and the mass of coupled water is evaluated by combination of QCM-D and SPR data. It turns out that adsorption can be tuned over a wide range (0.6–3.9 mg dry mass m–2) depending on the used conditions for adsorption and the type of employed cationic cellulose. After evaluation of protein adsorption, patterned cellulose thin films have been prepared and the cationic celluloses were adsorbed in a similar fashion as in the QCM-D and SPR experiments. Onto these cationic surfaces, fluorescently labeled BSA in different concentrations is deposited by an automatized spotting apparatus and a correlation between the amount of the deposited protein and the fluorescence intensity is established.

Publication year: 2014
Authors: Mohan T. 1, Niegelhell K. 4, Pinto Zarth C.S. 2, Kargl R. 3, Köstler S. 5, Ribitsch V. 1, Heinze T. 2, Spirk S. 1,4, Stana-Kleinschek K. 3

1 – Institute for Chemistry, University of Graz, Heinrichstrasse 28, 8010 Graz, Austria
2 – Institute for Organic Chemistry and Macromolecular Chemistry, Centre of Excellence for Polysaccharide Research, Friedrich Schiller University of Jena, Jena,Germany
3 – Institute for the Engineering and Design of Materials, University of Maribor, Maribor, Slovenia
4 – Institute for Chemistry and Technology of Materials, Graz University of Technology, Graz, Austria
5 – Joanneum Research Materials, Institute for Surface Technologies and Photonics, Weiz, Austria

Published in: Biomacromolecules, 2014, Vol. 15 (11), p. 3931–3941
DOI: 10.1021/bm500997s


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