Structural and Functional Characteristics of Chimeric Avidins Physically Adsorbed onto Functionalized Polythiophene Thin Films

Stabilized bioreceptor layers are of great importance in the design of novel biosensors. In earlier work, chimeric avidins enabled immobilization of biotinylated anti-bodies onto gold surfaces with greater stability compared to more conventional avidins (wild-type avidin and streptavidin). In the present study, the applicability of chimeric avidins as a general binding scaffold for biotinylated antibodies on spin-coated functionalized polythiophene thin films has been studied by surface plasmon resonance and atomic force microscopy. Novel chimeric avidins showed remarkably increased binding characteristics compared with other avidins, such as wild-type avidin, streptavidin, and bacterial avidin when merely physically adsorbed onto the polythiophene surface. They gave the highest binding capacities, the highest affinity constant, and the highest stability for biotinylated probe immobilization. Introduction of carboxylic acid groups to polythiophene layer further enhanced the binding level of the avidins. Polythiophene layers functionalized with chimeric avidins thus offered a promising generic platform for biosensor applications.

Publication year: 2012
Authors: W.M. Albers 1, J.M. Pelto 1, C. Suspenè 2, J.A.Määttä 3, A.Yassar 4, V.P. Hytönen 3,5, I.M.Vikholm-Lundin 1, K. Tappura 1

1 – VTT Technical Research Centre of Finland, Sinitaival 6 FI-33720 Tampere, Finland
2 – Laboratoire Itodys – UMR 7086, Universitè Paris Diderot, Universitè Paris 7 Diderot – Batiment Lavoisier, 15 rue Jean-Antoine de Baïf, 75205 Paris, France
3 – IBT Institute of Biomedical Technology, University of Tampere, BioMediTech and Tampere University Hospital, Biokatu 6, 33014, University of Tampere, Finland
4 – LPICM, UMR 7647 CNRS, Ecole Polytechnique, Route de Saclay, 91128, Palaiseau Cedex, France
5 – Centre for Laboratory Medicine, Tampere University Hospital, 33520 Tampere, Finland

Published in: ACS Applied Materials and Interfaces, 2012, Vol. 4, p. 4067 − 4077


antibody immobilization biosensor C-reactive protein chimeric avidin polythiophene refractive index thickness measurement


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