SPR-Based Detection of ASF Virus in Cells

African swine fever (ASF) is one of the most dangerous hemorrhagic infectious diseases that affect domestic and wild pigs. Currently, neither a vaccine nor effective treatments are available for this disease. As regards the degree of virulence, ASFV strains can be divided into high, moderate, or low virulence. The main detection methods are based on the use of the polymerase chain reaction (PCR). In order to prevent an uncontrolled spread of ASF, new on-site techniques that can enable the identification of an early-stage disease are needed. We have developed a specific immunological SPR-based assay for ASFV antigen detection directly in liquid samples. The developed assay allows us to detect the presence of ASFV at the dose of 103 HAD50/mL.

Publication year: 2022
Authors: Capo A. 1 2, Calabrese A. 1 2, Frant M. 3, Walczak M. 3, Szczotka-Bochniarz A. 3, Manessis G. 4, Bossis I. 5, Staiano M. 1, D'Auria S. 6, Varriale A. 1 2
  1. Institute of Food Science, CNR Italy, 83100 Avellino, Italy.
  2. URT-ISA, CNR at Department of Biology, University of Naples Federico II, 80126 Napoli, Italy.
  3. Department of Swine Diseases, National Veterinary Research Institute (NVRI), al. Partyzantow 57, 24-100 Pulawy, Poland.
  4. Laboratory of Anatomy and Physiology of Farm Animals, Department of Animal Science, Agricultural University of Athens (AUA), Iera Odos 75 Str., 11855 Athens, Greece.
  5. Laboratory of Animal Husbandry, Department of Animal Production, School of Agriculture, Faculty of Agriculture, Forestry and Natural Environment, Aristotle University of Thessaloniki, 54124 Thessaloniki, Greece.
  6. Department of Biology, Agriculture, and Food Sciences, National Research Council of Italy (CNR-DISBA), Piazzale Aldo Moro 7, 00185 Rome, Italy.
Published in: International Journal of Molecular Sciences, 2022, Vol 23, p. 7463
DOI: 10.3390/ijms23137463


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