Dispersive solid-phase imprinting of proteins for the production of plastic antibodies
We describe a novel dispersive solid-phase imprinting technique for the production of nano-sized molecularly imprinted polymers (nanoMIPs) as plastic antibodies. The template was immobilized on in-house synthesized magnetic microspheres instead of conventional glass beads. As a result, high-affinity and template-free MIPs were produced in higher yields.
Publication year: 2018
Authors: Ashley J., Feng X., Halder A., Zhou T., Sun Y.*
Affiliations:
Department of Micro and Nanotechnology, Technical University of Denmark, Ørsteds Plads, Lyngby, Denmark
* corresponding author
Published in: Chemical Communications, 2018, Vol. 54 (27), p. 3355-3358
DOI: 10.1039/c8cc00343b
MP-SPR KEYWORDS
affinity
b-lactoglobulin
binding
hemoglobin
molecularly imprinted polymers
protein-nanoMIP interaction
RNase
trypsin