Control of Protein Affinity of Bioactive Nanocellulose and Passivation Using Engineered Block and Random Copolymers

We passivated TEMPO-oxidized cellulose nanofibrils (TOCNF) toward human immunoglobulin G (hIgG) by modification with block and random copolymers of poly(2-(dimethylamino)ethyl methacrylate) (PDMAEMA) and poly(oligo(ethylene glycol) methyl ether methacrylate) (POEGMA). The block copolymers reversibly adsorbed on TOCNF and were highly effective in preventing nonspecific interactions with hIgG, especially if short PDMAEMA blocks were used. In such cases, total protein rejection was achieved. This is in contrast to typical blocking agents, which performed poorly. When an anti-human IgG biointerface was installed onto the passivated TOCNF, remarkably high affinity antibody-antigen interactions were observed (0.90 ± 0.09 mg/m(2)). This is in contrast to the nonpassivated biointerface, which resulted in a significant false response. In addition, regeneration of the biointerface was possible by low pH aqueous wash. Protein A from Staphylococcus aureus was also utilized to successfully increase the sensitivity for human IgG recognition (1.28 ± 0.11 mg/m(2)). Overall, the developed system based on TOCNF modified with multifunctional polymers can be easily deployed as bioactive material with minimum fouling and excellent selectivity.

Publication year: 2016
Authors: Vuoriluoto M. 1, Orelma H. 1, Zhu B. 2, Johansson L.S. 1, Rojas O.J. 1

1 – Biobased Colloids and Materials (BiCMat), Department of Forest Products Technology, School of Chemical Technology, Aalto University , FI-00076, Espoo, Finland
2 – DWI – Leibniz-Institute for Interactive Materials Research , Forckenbeckstr. 50, D-52056 Aachen, Germany

Published in: ACS Applied Materials and Interfaces, 2016, Vol. 8, Issue 8, p: 5668-78
DOI: 10.1021/acsami.5b11737


adsorption antibody-antigen interaction on cellulose surface biointerface block copolymer cellulose in situ TEMPO-oxidation molecule material interaction protein adsorption on TOCNF regeneration resistant surface surface functionalization


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